Pre-1945 state roads 21-38

One useful source is a 1939 list published by the SRD. I had previously taken notes on all the laws, but this combines them in one handy booklet.

Note that many of the legislatively-defined routes were never built or never acquired by the state. This is especially true of higher numbers.

#	year	from	to	via (post-1945)
21	1923	140 in Daytona Beach	Eustis	1 in Daytona Beach (now 600) 600 Daytona Beach to DeLand 44 DeLand to Eustis
	only went from Daytona to DeLand in 1923; extended west to Eustis in 1927 and east across the Intracoastal Waterway to 140 in 1937
22	1923	Crystal River	Indian River City	44 Crystal River to Inverness 45 Inverness to Floral City 48 Floral City to Center Hill 469 Center Hill to east of Mabel 50 east of Mabel to Tildenville 438 Tildenville to Ocoee 439 Ocoee to Minorville 50 Minorville to Indian River City
23	1923	Rerdell	Palmetto	35 Rerdell to Dade City 39 Dade City to Plant City 574 Plant City to Tampa 45 Tampa to Palmetto
	went from Belleview to Plant City in 1923; extended north to Ocala and south to Palmetto in 1927; north of Rerdell was probably never deleted, but it became part of 2 in 1931, and maps show it as only 2
23	1931	Sumterville	Rutland	470
	also part of 225
23	1931	Bushnell	Webster	48 Bushnell to Bevilles Corner 471 Bevilles Corner to Webster
	also parts of 22 and 2
24	1923	Mount Plymouth	Indialantic	435 Mount Plymouth to Apopka 437A Apopka to Paradise Heights 437 Paradise Heights to Fullers Fullers Cross Road Fullers to Winter Garden 535 Winter Garden to south of Vineland 530 south of Vineland to Kissimmee 500 Kissimmee to Melbourne 516 Melbourne to Indialantic (now 500)
	only went from Kissimmee to Melbourne in 1923; extended to Indialantic in 1933 and to Mount Plymouth in 1935
25	1923	140 in Palm Beach	Captiva	80 Palm Beach to Fort Myers 867 Fort Myers to Punta Rassa Sanibel-Captiva Road Sanibel to Captiva
	ran from West Palm Beach to Olga (on 2) in 1923; extended east from Military Trail to 140 in 1929, west to Punta Rassa in 1931, and to Captiva in 1935
26	1923	South Bay	Fort Lauderdale	25 South Bay to Andytown 84 Andytown to Fort Lauderdale
	defined in 1923 to run from 8 near Childs to 25 (near Clewiston); extended southeast to Fort Lauderdale in 1925; original piece also numbered 67 in 1927, and removed from 26 by 1929 (specifically from Childs to Clewiston); since Clewiston to South Bay was 25, the SRD eventually considered 26 to begin at South Bay branch added in 1929 along Miami Canal from northwest of Hialeah to 25 (at Lake Harbor); removed in 1931
26	1927	Andytown	Miami	25
27	1923	Fort Myers	Miami	45 Fort Myers to Naples 90 Naples to Miami
27	1939	east of Carnestown	west of Miami	94
27-A	1931	Royal Palm Hammock	Collier City	92
	bill was passed in 1929 but was mislaid and so didn't become a law until 1931
28	1923	Lake City	Flagler Beach	100 Lake City to Bunnell 11 Bunnell to Flagler Beach (now 100)
	went from Lake City to Starke in 1923; extended to Palatka and then to Bunnell in 1925, and to Flagler Beach in 1937 (replacing 72, defined in 1927)
29	1925	Orlando	25 near Clewiston	15 Orlando to Okeechobee 78 Okeechobee to Moore Haven 721 Moore Haven to Clewiston
	29 was defined in 1923 from Perry to Branford; this became part of 5-A in 1925 29 went from Bithlo to Moore Haven in 1925; the north end was truncated to Holopaw in 1929 and extended to Orlando in 1931; the south end was extended to Clewiston in 1935 (along what had been part of 26 from 1923 to 1929 (?) and 67 from 1927 to 1931)
29	1929	Okeechobee	Jupiter	710 Okeechobee to Indiantown Old Indiantown Road Indiantown to west of Jupiter 706 west of Jupiter
30	1923	Ruskin	Vero Beach	674 Ruskin to Brewster 630 Brewster to Fort Meade 700 Fort Meade to Frostproof 630 Frostproof to west of Kissimmee River 60 west of Kissimmee River to Vero Beach
	defined in 1923 as Frostproof to Vero Beach; extended west to Fort Meade in 1933, Brewster in 1935, and Ruskin in 1937
31	1923	Ocala	Waldo	200
32	1923	Bradenton	30 west of Kissimmee River	45 Bradenton to Palmetto 43 Palmetto to Parrish 62 Parrish to Wauchula 650 (now 636)/652 east of Wauchula 64 east of Wauchula to Avon Park Old Bombing Range Road/Arbuckle Road Avon Park to Fort Kissimmee unbuilt Fort Kissimmee to 30
	extended from Avon Park to 30 in 1927 maps show both 650 and 652 as 32; 652 is the older main road, while 650 was still unpaved in 1945
33	1923	Alabama	Crestview	85
34	1923	Duette	15 at Hernando-Citrus County line	37 Duette to Lakeland 35 Lakeland to Dade City 52 in Dade City 41 Dade City to north of Spring Lake 50 north of Spring Lake to Brooksville 485 Brooksville to 15 (now 700)
	ran from Dade City northwest to Brooksville in 1923; extended south to Lakeland in 1931 and Duette in 1935, and northwest to 15 in 1941
35	1923	Georgia	Keaton Beach	55 Georgia to Perry 361 Perry to Keaton Beach
	extended from Perry to Keaton Beach in 1935
35-A	1937	35 in Perry	500 in Perry	Green Street
36	1923	Leesburg	Inverness	44
37	1923	Alabama	Milton	87 Alabama to Milton 193 in Milton (North Avenue, Alabama Street, and Canal Street)
38	1923	Pedro	100 east of Altoona	42 Pedro to east of Starkes Ferry 450 east of Starkes Ferry to east of Altoona
	went from Weirsdale to Umatilla in 1923; extended west to Pedro in 1933 and east to 100 in 1935